Genotyping of toxigenic strains of Clostridium difficile
نویسندگان
چکیده
منابع مشابه
The Frequency of Toxigenic Strains of Clostridium difficile in Hospitalized Patients with Diarrhea in Tehran/Iran by PCR Method, 2010
Background & Aims: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea (AAD), pseudomembranous colitis and also nosocomial diarrhea. The present study was performed to find the prevalence of toxigenic strains of C .difficile isolated from diarrhea patients hospitalized in Tehran hospitals. Method: A total of 98 fecal samples obtained during July to December...
متن کاملIdentification of toxigenic Clostridium difficile by counterimmunoelectrophoresis.
A counterimmunoelectrophoresis (CIE) technique which reacted positively with culture filtrates of Clostridium difficile was developed and compared with a cytotoxicity assay in human embryonic lung cell cultures. CIE, employing C. sordellii antitoxin, detected 17 of 17 C. difficile strains. Of those positive by CIE, 13 were cytotoxic in cell culture. Fourteen Clostridium species other than C. di...
متن کاملCommunity Environmental Contamination of Toxigenic Clostridium difficile
BACKGROUND Clostridium difficile infection is often considered to result from recent acquisition of a C difficile isolate in a healthcare setting. However, C difficile spores can persist for long periods of time, suggesting a potentially large community environmental reservoir. The objectives of this study were to assess community environmental contamination of toxigenic C difficile and to asse...
متن کاملMolecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملSpecific detection of toxigenic strains of Clostridium difficile in stool specimens.
Clostridium difficile is the infectious agent responsible for antibiotic-associated colitis. We report the use of the polymerase chain reaction technique to identify toxigenic strains of C. difficile in human stool specimens. A set of primers based on the nucleotide sequence of the toxin B gene, which amplified a 399-bp fragment from isolates producing toxin B, was designed. We examined 28 know...
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ژورنال
عنوان ژورنال: Medicina Fluminensis
سال: 2018
ISSN: 1847-6864
DOI: 10.21860/medflum2018_203560